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Catalog Number:
41959
CAS Number:
58880-05-0
Ethidium bromide monoazide
Grade:
suitable for Hematology & Histology
Purity:
≥ 95% (HPLC)
Synonym(s):
3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide
Temperature Sensitive
Documents
$409.01 /5MG
Pack Size Availability Price
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Product Information

Ethidium bromide monoazide is a vital compound widely utilized in molecular biology and biochemistry for its unique properties as a nucleic acid stain. This compound is particularly effective in distinguishing between viable and non-viable cells, making it an essential tool in various applications such as flow cytometry and fluorescence microscopy. Ethidium bromide monoazide selectively penetrates dead cells, allowing researchers to accurately assess cell viability and apoptosis in a range of biological studies. Its ability to intercalate into DNA and RNA provides a reliable method for visualizing nucleic acids, facilitating the analysis of genetic material in clinical diagnostics and research settings.

Moreover, this compound is advantageous in applications involving PCR and gel electrophoresis, where it enhances the detection of nucleic acids. Ethidium bromide monoazide's unique azide group allows for further modifications, expanding its utility in advanced research applications. With its high sensitivity and specificity, this compound is invaluable for researchers seeking to explore cellular processes and genetic information, ensuring precise and reliable results in their experiments.

Synonyms
3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide
CAS Number
58880-05-0
Purity
≥ 95% (HPLC)
Grade
suitable for Hematology & Histology
Molecular Formula
C21H18BrN5
Molecular Weight
420.31
MDL Number
MFCD01708677
PubChem ID
2762649
Appearance
Orange solid
Conditions
Store at ≤ -10 °C
General Information
Synonyms
3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide
CAS Number
58880-05-0
Purity
≥ 95% (HPLC)
Grade
suitable for Hematology & Histology
Molecular Formula
C21H18BrN5
Molecular Weight
420.31
MDL Number
MFCD01708677
PubChem ID
2762649
Appearance
Orange solid
Conditions
Store at ≤ -10 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Ethidium bromide monoazide is widely utilized in research focused on:

  • Molecular Biology: This compound is commonly used in nucleic acid research, particularly for labeling DNA. Its ability to intercalate with nucleic acids allows for effective visualization during gel electrophoresis.
  • Cell Viability Assays: It serves as a vital tool in assessing cell viability. By distinguishing between live and dead cells, it aids researchers in evaluating the effects of drugs or treatments on cellular health.
  • Microbial Studies: Ethidium bromide monoazide is employed in studies to differentiate viable bacteria from non-viable ones. This is crucial in microbiology for understanding microbial populations in various environments.
  • Genetic Engineering: In genetic engineering applications, it helps in the selective labeling of nucleic acids, facilitating the study of gene expression and regulation in various organisms.
  • Forensic Science: The compound is also valuable in forensic applications, particularly in analyzing DNA samples from crime scenes, ensuring accurate identification of biological materials.

Citations