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Catalog Number:
20972
CAS Number:
135313-63-2
4-Chloro-3-indolyl β-D-galactopyranoside
Synonym(s):
4-Chloro-3-indoxyl-β-D-galactopyranoside
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Product Information

4-Chloro-3-indolyl b-D-galactopyranoside is a valuable compound widely utilized in biochemical research, particularly in the study of enzyme activity and gene expression. This compound serves as a chromogenic substrate for β-galactosidase, enabling researchers to visualize enzyme activity through colorimetric assays. Its unique structure, featuring a chloroindole moiety, enhances its reactivity and specificity, making it an excellent choice for applications in molecular biology and biochemistry.

In addition to its role in enzyme assays, 4-Chloro-3-indolyl b-D-galactopyranoside is also employed in the development of biosensors and diagnostic tools, where precise detection of β-galactosidase activity is crucial. Its ability to produce a distinct color change upon enzymatic cleavage allows for easy monitoring and quantification, providing researchers with reliable data for their experiments. This compound's versatility and effectiveness in various applications make it an essential reagent for laboratories focused on enzyme research and related fields.

Synonyms
4-Chloro-3-indoxyl-β-D-galactopyranoside
CAS Number
135313-63-2
Molecular Formula
C14H16ClNO6
Molecular Weight
329.73
MDL Number
MFCD00040638
PubChem ID
3626840
Conditions
Store at 0-8°C
General Information
Synonyms
4-Chloro-3-indoxyl-β-D-galactopyranoside
CAS Number
135313-63-2
Molecular Formula
C14H16ClNO6
Molecular Weight
329.73
MDL Number
MFCD00040638
PubChem ID
3626840
Conditions
Store at 0-8°C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

4-Chloro-3-indolyl b-D-galactopyranoside is widely utilized in research focused on:

  • Biochemical Assays: This compound serves as a substrate in various enzymatic assays, particularly for measuring β-galactosidase activity, which is crucial for studying gene expression and enzyme kinetics.
  • Cell Biology: It is employed in cell culture experiments to track cellular processes, such as differentiation and apoptosis, by providing a visual marker that indicates enzyme activity.
  • Drug Development: In pharmaceutical research, it aids in the screening of potential drug candidates by evaluating their effects on specific biological pathways, particularly those involving carbohydrate metabolism.
  • Genetic Research: This compound is used in molecular biology to study gene regulation and expression, especially in systems involving lactose operons, making it valuable for genetic engineering applications.
  • Food Industry: It finds applications in food science for assessing the activity of enzymes involved in lactose breakdown, which can improve dairy product formulations and enhance lactose-free options.

Citations