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Catalog Number:
02619
CAS Number:
28529-34-2
Acetyl-L-leucine amide
Purity:
≥ 99% (TLC)
Synonym(s):
Ac-L-Leu-NH2
Documents
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Product Information

Acetyl-L-leucine amide (N-acetyl-L-leucinamide) is a small, chiral peptide derivative with the molecular formula C8H16N2O2 and a molecular weight of 172.22 g/mol. Structurally, it consists of an L-leucine backbone bearing an N-terminal acetyl group and a C-terminal primary carboxamide, resulting in a neutral, non-ionic amide-amide framework. The presence of both secondary and primary amide functional groups provides defined hydrogen-bond donor and acceptor sites, while the isobutyl side chain contributes a hydrophobic character. This balance of polar and nonpolar features, together with a total polar surface area of 72.2 Ų, supports predictable behavior in aqueous and mixed organic solvent systems commonly used in analytical workflows.

As an N-acylated amino acid amide, acetyl-L-leucine amide is a useful model compound for studying peptide-like recognition, amide bond stability, and conformational preferences in solution. Its defined stereochemistry at the α-carbon allows investigation of chiral interactions in separation science and binding assays. The molecule’s dual amide functionality makes it relevant for probing hydrogen-bonding patterns in spectroscopic and chromatographic method development. In addition, its simple, well-characterized structure makes it suitable as a reference or test analyte in method validation for peptide and amino-acid-related analyses.

Synonyms
Ac-L-Leu-NH2
CAS Number
28529-34-2
Purity
≥ 99% (TLC)
Molecular Formula
C8H16N2O2
Molecular Weight
172.2
MDL Number
MFCD00026506
PubChem ID
4092029
Melting Point
129 - 135 °C
Appearance
White powder
Optical Rotation
[a]24D = -21 ± 1 °
Conditions
Store at 0 - 8 °C
General Information
Synonyms
Ac-L-Leu-NH2
CAS Number
28529-34-2
Purity
≥ 99% (TLC)
Molecular Formula
C8H16N2O2
Molecular Weight
172.2
MDL Number
MFCD00026506
PubChem ID
4092029
Melting Point
129 - 135 °C
Appearance
White powder
Optical Rotation
[a]24D = -21 ± 1 °
Conditions
Store at 0 - 8 °C
Properties
Additional property information coming soon!
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Safety and Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Warnings
-
Applications

Acetyl-L-leucine amide is a peptide-like model compound suitable for use in analytical method development, chiral studies, and investigations of amide bond behavior in solution.

  • Model peptide analog in spectroscopic studies Used to investigate amide bond conformation and hydrogen bonding by NMR, IR, or UV-based techniques due to its dual amide functionalities and defined stereocenter.
  • Chiral chromatography method development Serves as a test analyte for optimizing chiral stationary phases and mobile phase conditions, exploiting its single L-configuration stereocenter and modest hydrophobicity.
  • Reference compound for peptide-like LC-MS workflows Acts as a low-molecular-weight standard for tuning retention, ionization, and fragmentation parameters in liquid chromatography–mass spectrometry analyses of peptide-related compounds.
  • Hydrogen-bonding and solvation studies Provides a simple framework for examining solvent effects and hydrogen-bond networks involving secondary and primary amide groups in mixed aqueous-organic systems.
  • Enzyme and recognition model substrate Functions as a structurally defined probe for assessing specificity of amide- or peptide-recognizing proteins and synthetic receptors without complications from ionizable termini.
  • Calibration standard in amide quantitation assays Can be employed as a structurally simple amide-containing standard for developing and validating quantitative analytical methods targeting amide-bearing analytes.

Citations