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Catalog Number:
21763
CAS Number:
1158-17-4
o-Nitrophenyl-1-thio-β-D-galactopyranoside
Purity:
≥ 99% (HPLC)
Synonym(s):
2-Nitrophenyl 1-thio-β-D-galactopyranoside, o-Nitrophenylsulfenyl-1-thio-β-D-galactoside
Temperature Sensitive
Documents
$58.39 /100MG
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Product Information

o-Nitrophenyl-1-thio-b-D-galactopyranoside is a valuable compound widely utilized in biochemical research, particularly in the study of glycosidases and enzyme kinetics. This substrate is recognized for its ability to release o-nitrophenol upon hydrolysis, making it an effective tool for measuring enzyme activity in various assays. Its specificity for β-galactosidase allows researchers to investigate the enzyme's properties and behavior in a controlled environment, providing insights into metabolic pathways and potential therapeutic targets.

In addition to its applications in enzyme assays, o-Nitrophenyl-1-thio-b-D-galactopyranoside serves as a crucial reagent in the synthesis of glycosides and other carbohydrate derivatives. Its unique structure, featuring a nitrophenyl group, enhances its reactivity and makes it a preferred choice for researchers looking to explore carbohydrate chemistry. The compound's stability and ease of use further contribute to its appeal, allowing for reliable results in both academic and industrial settings.

Synonyms
2-Nitrophenyl 1-thio-β-D-galactopyranoside, o-Nitrophenylsulfenyl-1-thio-β-D-galactoside
CAS Number
1158-17-4
Purity
≥ 99% (HPLC)
Molecular Formula
C12H15NO7S
Molecular Weight
317.32
MDL Number
MFCD00076099
PubChem ID
5162107
Melting Point
190 - 200 °C
Appearance
Yellow crystalline powder
Optical Rotation
[a]20D = - 250 to 256 º (C=0.7 in MeOH)
Conditions
Store at ≤ -4 °C
Safety & Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
General Information
Synonyms
2-Nitrophenyl 1-thio-β-D-galactopyranoside, o-Nitrophenylsulfenyl-1-thio-β-D-galactoside
CAS Number
1158-17-4
Purity
≥ 99% (HPLC)
Molecular Formula
C12H15NO7S
Molecular Weight
317.32
MDL Number
MFCD00076099
PubChem ID
5162107
Melting Point
190 - 200 °C
Appearance
Yellow crystalline powder
Optical Rotation
[a]20D = - 250 to 256 º (C=0.7 in MeOH)
Conditions
Store at ≤ -4 °C
Safety & Regulations
Hazmat
No
Antibiotic
No
DEA-regulated
No
Properties
Additional property information coming soon!
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Applications

o-Nitrophenyl-1-thio-b-D-galactopyranoside is widely utilized in research focused on:

  • Enzyme Activity Assays: This compound serves as a substrate for various glycosidases, allowing researchers to measure enzyme activity in biochemical assays. Its ability to produce a color change upon hydrolysis makes it an effective tool for quantifying enzyme kinetics.
  • Biochemical Research: It is commonly used in studies involving carbohydrate metabolism and enzyme mechanisms, providing insights into how enzymes interact with substrates in biological systems.
  • Diagnostic Applications: The compound can be employed in diagnostic tests to identify specific enzyme deficiencies or activities in clinical samples, aiding in the diagnosis of metabolic disorders.
  • Drug Development: In pharmaceutical research, it helps in screening potential drug candidates that target glycosidases, which are important in various diseases, including cancer and infections.
  • Educational Purposes: It is often used in laboratory settings for teaching purposes, helping students understand enzyme-substrate interactions and the principles of enzymology through hands-on experiments.

Citations